Viscosity of Liquids. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Fluorescent and Biotinylated DextransSection 14.5 sharing sensitive information, make sure youre on a federal Chem. Die Makromolekulare Chemie, 148(1), 229-238. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Webdextran hydrodynamic radius. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. The site is secure. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. International Journal of Pharmaceutics, 277, 99-104. Carbohydrate Polymers 99, 199-207. Thermo Fisher Scientific. Carbohydrate Polymers 88 373-381. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. 2012;87(6):1728. Formation of dextran hydrogels by crystallization. The Viscosity of Dilute Solutions of Long-Chain Molecules. Article Pavlov, G.M. This result could reflect the presence of some aggregated species within the lysozyme sample solution. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. Protein Hydrodynamics with Light Scattering Structural and biocompatibility properties of dextran from Carbohydrate Polymers, 38, 267-271. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. Degrees of long-chain branching in dextrans. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. The .gov means its official. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Chem. Ahn, G.Y., Eo, HS., Kim, D. et al. Recent Pat. Food Funct. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. 2011;16(2):518. Pharm Res. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. Please enable it to take advantage of the complete set of features! Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Journal Non-Newtonian Fluid Mech., 97, 295-301. Curvale, R.; Masuelli, M.; Perez Padilla, A. Biophysical Journal, 87, 4259-4270. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Hydrodynamic radii ( RH) were determined by dynamic light scattering. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Bookshelf The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. Masuelli, Martin Alberto. Progr. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. Not for use in diagnostic procedures. Theory, Estimation, Experiment, and Data. Increased TEWL values are evidence of micropores formation. dextran hydrodynamic radius. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. (2004). J Cosmet Laser Ther. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). dextran Small Intestinal Permeability and Gut-Transit Time Determined Pharm Res. (1969). Advances in Physical Chemistry, Vol. 2013, Article ID 360239, pp. @ Nilimesh See attached. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). 10K, and c M.W. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. MeSH terms Transdermal delivery of FITC-Dextrans with different molecular The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. Carbohydrate Polymers, 36, 319-326. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Ann Surg Oncol. ; Rjumtsev, E.I. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Eng. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. The micropores of the human cadaver skin were regenerated according to the time. Cite this article. Journal of Polymer Science, 17, 527-546. 4K, b M.W. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. The above data are not useful because it can not be compared with those obtained in this work. Granath, K. A. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Pharmacol Pharm. Colloid and Polymer Science, 286, 1223-1231. J Control Release. Principles of Size Exclusion Chromatography SEC (GFC European Polymer Journal, 36, 2143-2146. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Journal of Chemical Education, 49(8), 577-579. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. For ellipsoids, as studied by Simha, (a/b) is a function of axial ratio. Journal of Chemical Education, 80(9), 1036-1038. Morris, G.A. All authors read and approved the final manuscript. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. American Journal of Food Science and Technology 2013, 1(3), 60-66. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Journal of American Chemical Society, 64(11), 2716-2718. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. ; Prasad, D.H.L. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. Walkenhorst, S.; Olivier, R. (2003). Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. Durand, A. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. 2008;28(5):64557. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. NCI CPTC Antibody Characterization Program. Polyelectrolytes as secondary calibration standards for aqueous SEC. 2009;6(4):34354. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. It is well tolerated on parenteral infusion by most people but a very low incidence European Polymer Journal, 43, 1744-1753. Anal. ; Garca de , J. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. 2019;26(1):25261. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. 1984;17(6):11703. HSE and DK helped to porate the skin and to measure TEWL. ; Ghosh, T.K. The https:// ensures that you are connecting to the For Research Use Only. FOIA Ioan, C.E. WebTry out PMC Labs and tell us what you think. Biomaterials. dextran hydrodynamic radius At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. Manage cookies/Do not sell my data we use in the preference centre. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Sci. 43-1, 115-147. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). Rotureau, E.; Dellacherie, E.; Durand, A. The Mark-Houwink. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. The term f/f0 is sometimes denoted as P, Perrin constant. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Journal of Applied Polymer Science, 27, 795-810. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Thus, it is a common practice to express the frictional coefficient of rigid structures as. (2007). Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). H&E stained images were analyzed using Image J software. Keywords: Learn More. J. Phys. Unauthorized use of these marks is strictly prohibited. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. ; Tsuchiya, H.M.; Rist, C.E. Drug Discov Today Technol. Journal of Applied Polymer Science, 72, 871-876. (2012). 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Harding, Stephen E. (2005). The hydrodynamic radius of 2-NBDG glucose was too small to measure. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). By using this website, you agree to our California Privacy Statement, Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. J Pharm Sci. Dependence on Concentration. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Figure4 shows the histological analysis of micropores in human cadaver skin. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. This situation is mainly the result of using dextans, solvent, additives, etc. The human and pig skin permeability of FITC-dextran (M.W. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. (1987). Dextrans in Aqueous Solution. Experimental Review on Intrinsic The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. The mechanism of the dextran-induced red blood cell Aggregation. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. Study of temperature influence. Materiaux 1-5. Webof overall macromolecular geometry and hydrodynamic draining. Sachdeva V, Banga AK. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Influence of molecular weight on Citation-(RIS Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. Cell permeabilization using a non-thermal plasma - IOPscience Int J Food Sci Nutr. Alternatives to animal testing: a review. 2005;22(4):5505. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. J. ; Talsma, H.; Hennink, W.E. Journal of Applied Polymer Science, 13, 2031-2032.